int piezoPin = 2;   // buzzer connect to pin 2
int ledPin = 13;    // LED connect to pin 13

void setup() {

  pinMode(piezoPin, OUTPUT);
  pinMode(ledPin, OUTPUT);

}

void loop() {

  digitalWrite(ledPin, HIGH);

  tone(piezoPin, 300);    // no data
  delay(60000);
  noTone(piezoPin);
  delay(5000);

  tone(piezoPin, 400);    // germination index, height of stem, activity of root system and the penetrability of cell membrane
  delay(60000);
  noTone(piezoPin);
  delay(5000);

  tone(piezoPin, 500);    // no data
  delay(60000);
  noTone(piezoPin);
  delay(5000);

  tone(piezoPin, 1000);   // no data
  delay(60000);
  noTone(piezoPin);
  delay(5000);

  tone(piezoPin, 20000);  // gene expression modulation
  delay(60000);
  noTone(piezoPin);
  delay(5000);

  digitalWrite(ledPin, LOW);

}